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Fig. 7 | BMC Chemical Engineering

Fig. 7

From: Integration of aqueous (micellar) two-phase systems on the proteins separation

Fig. 7

Diagram of the integrated process to selectively separate cytochrome c (Cyt c), ovalbumin (Ova) and azocasein (Azo). The proposed strategy includes two steps of purification using, respectively, ATPS and AMTPS based on Pluronic L-35 and potassium phosphate buffer - PB (pH = 6.6). The purity (Px) and recovery (Rx) of each step is provided in the present diagram. An ultrafiltration was applied to improve the separation of Cyt c and Ova and an acid precipitation was applied to isolate the Azo from Pluronic L35. The potassium phosphate buffer was maintained in the Azo-rich phase as a stabilizing solution

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