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Table 3 Qualitative estimation of lipid classes by specific reagents following separation by TLC [78]

From: Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art

Lipid class Reagent Results
Free fatty acids 2′,7′-Dichlorofluorescein/ AlCl3/FeCl3 Appears as rose color after few minutes
Glycolipids α-Naphthol/sulfuric acid Yellow spots
Orcinol/sulfuric acid Blue-purple spots
5-Hydroxy-1-tetralone in 80% sulfuric acid Glycolipids give yellow spots easily distinguishable from the light blue spots of phospholipids
Sphingolipids Sodium hypochlorite/benzidine reagent Blue spots on white background
Phospholipids Molybdic oxide/molybdenum Blue spots on white background
Cholesterol and cholesteryl esters Acidic ferric chloride Red to violet spots