Lipid class | Reagent | Results |
---|---|---|
Free fatty acids | 2′,7′-Dichlorofluorescein/ AlCl3/FeCl3 | Appears as rose color after few minutes |
Glycolipids | α-Naphthol/sulfuric acid | Yellow spots |
Orcinol/sulfuric acid | Blue-purple spots | |
5-Hydroxy-1-tetralone in 80% sulfuric acid | Glycolipids give yellow spots easily distinguishable from the light blue spots of phospholipids | |
Sphingolipids | Sodium hypochlorite/benzidine reagent | Blue spots on white background |
Phospholipids | Molybdic oxide/molybdenum | Blue spots on white background |
Cholesterol and cholesteryl esters | Acidic ferric chloride | Red to violet spots |