Standing-wave Design of Three-Zone, open-loop non-isocratic SMB for purification

Table 3 Results for Example 1: Comparison with Optimal Batch

Parameter	Separation of Target IgG from a Weakly Adsorbing Impurity
Parameter	Case 1	Case 2	Case 3	Optimal Batch
Column length (cm)	2.5	2.5	2.5	8.5
Pressure limit (kPa)	250	690	2580^a	250
Number of columns	3	3	3	1
Flow rates (mL/min)	0.78/0.25/15.3	2.1/0.68/41.7	9.2/2.9/178	6.5
Flow rates (mL/min)	(I/II/III)	(I/II/III)	(I/II/III)	6.5
Step time (s), pH-SMB	121	44	10
Cycle time (s)	362	132	30	450
Product conc. (g/L)	19	19	19	4.64
Solvent Consumption	0.05	0.05	0.05	0.4
(L extract/L feed)	0.05	0.05	0.05	0.4
Purity	99.9	99.9	99.9	98.1
Yield	99.4	99.2	99.4	97.7
Productivity (kg/L/day)	15	40	171	2.9
Normalized Productivity	5.2	13.8	59.0	1

^aNo pressure limit was set in this case. Pressure was calculated using the Ergun Equation [42]

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