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Table 3 Results for Example 1: Comparison with Optimal Batch

From: Standing-wave Design of Three-Zone, open-loop non-isocratic SMB for purification

Parameter Separation of Target IgG from a Weakly Adsorbing Impurity
Case 1 Case 2 Case 3 Optimal Batch
Column length (cm) 2.5 2.5 2.5 8.5
Pressure limit (kPa) 250 690 2580a 250
Number of columns 3 3 3 1
Flow rates (mL/min) 0.78/0.25/15.3 2.1/0.68/41.7 9.2/2.9/178 6.5
(I/II/III) (I/II/III) (I/II/III)
Step time (s), pH-SMB 121 44 10  
Cycle time (s) 362 132 30 450
Product conc. (g/L) 19 19 19 4.64
Solvent Consumption 0.05 0.05 0.05 0.4
(L extract/L feed)
Purity 99.9 99.9 99.9 98.1
Yield 99.4 99.2 99.4 97.7
Productivity (kg/L/day) 15 40 171 2.9
Normalized Productivity 5.2 13.8 59.0 1
  1. aNo pressure limit was set in this case. Pressure was calculated using the Ergun Equation [42]