Table 4 Results for Example 2: Comparison with Literature pH-SMB for the Separation of Target antibody fragments from a competitive adsorbing impurity
From: Standing-wave Design of Three-Zone, open-loop non-isocratic SMB for purification
Parameter | Case 1 | Case 2 | Case 3 | Literature pH-SMB |
|---|---|---|---|---|
Column length (cm)a | 2.5 | 2.5 | 1 | 2.5 |
Resin Pressure Limit (kPa) | 500 | 500 | 500 | 500 |
Particle Size (μm) | 34 | 80 | 34 | 34 |
Number of columns | 3 | 3 | 3 | 4 |
Flow rates (mL/min) | 12.0/13.1/36.1 | 86.7/84.1/161.1 | 27.9/30.8/60.8 | 1.03/1.02/2.3 |
(I/II/III) | (I/II/III) | (I/II/III) | (I/II/III) | |
Step time (s), pH-SMB | 61 | 10 | 11 | 282–324 |
Cycle time (s) | 182 | 30 | 33 | 1128-1269 |
Product conc. (mg/L) | 1.7 | 0.78 | 0.95 | 1.1 |
Solvent consumption | 0.52 | 1.12 | 0.93 | 0.44 |
(L Extract/L Feed) | ||||
Purity | 99 | 99 | 99 | 57–66 |
Yield | 99 | 99 | 99 | 27.4–91.4 |
Productivity (g/L/day) | 10 | 34 | 37 | 0.17–0.58 |
Normalized Productivity | 17–59 | 58–199 | 64–220 | 1 |